Research Assistant II

• Perform fluorescence-activated cell sorting (FACS), including aseptic sorting of human and mouse samples, and monitor sort purity and recovery
• Perform daily quality control, laser alignment, and calibration (e.g., with standardized beads), plus maintenance and troubleshooting, on our high-speed cell sorters and benchtop analyzers
• Advise investigators on experimental design, including multicolor panel design, compensation, and controls (e.g., FMO, viability dye)
• Analyze flow cytometry data (e.g., FlowJo) and assess data quality, and help investigators with gating strategy and presentation of results
• Train new researchers and staff with instrument operation and good flow cytometry practice, and provide ongoing support
• Handle billing, invoicing, and day-to-day lab operations
• Stay current in the field by attending vendor and technical training, participating in journal club, and giving presentations on flow cytometry methods and new technologies.

• Bachelor's degree in biological or physical sciences (or related field) plus 2+ years of laboratory and flow cytometry experience in a clinical, industry, or academic setting, or a master degree in a related field

• Hands-on experience with multicolor flow cytometry; cell sorting experience preferred
• Working knowledge of biosafety practices for handling human-derived samples (BSL-2)
• Ability to work independently, with strong problem-solving, attention to detail, and written and verbal communication
• Proficiency with PC and Mac platforms and Microsoft Office
• Comfortably working in a diverse, multicultural research environment and is a team player
• Ability to safely lift 50 lbs. (with or without accommodation)

The posted pay range is Boston Children's reasonable and good-faith expectation for this pay at the time of posting.

Any base pay offer provided depends on skills, experience, education, certifications, and a variety of other job-related factors. Base pay is one part of a comprehensive benefits package that includes flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.