Research Associate, Proteomics (#1909)

BACKGROUND

The National Biodefense Analysis and Countermeasures Center (NBACC) is a unique facility located at Fort Detrick in Frederick, MD, dedicated to defending the nation against biological threats. Established in response to biodefense gaps identified after the Amerithrax attacks of 2001, NBACC has been operated by the Battelle National Biodefense Institute (BNBI) since 2006.

NBACC supports DHS and national biodefense preparedness planning, response, emerging threat characterization, and bioforensic analyses. It addresses critical scientific knowledge gaps regarding biological agents to protect the public and defend the nation against biological threats- whether naturally occurring, accidental, or deliberate. NBACC also provides federal law enforcement with scientific data to support the investigation and attribution of biocrimes and the protection of the U.S. bioeconomy.

NBACC is comprised of two centers:

• National Bioforensic Analysis Center (NBFAC): Conducts technical analyses to support federal law enforcement investigations.
• National Biological Threat Characterization Center (NBTCC): Conducts experiments and studies to gather data for understanding biological vulnerabilities and hazards.

Together, these centers serve as a national resource for assessing risks posed by biological agents and emerging technologies, informing biodefense policy and response planning, and supporting the investigation, prosecution, and prevention of biocrimes and bioterrorism.

RESPONSIBILITIES

• Participates in the development, validation, and conduct of mass spectrometry and proteomics methods for identifying and characterizing biological threat agents including proteins and small molecular weight toxins.
• Operates, maintains, and troubleshoots lab equipment such as mass spectrometers (orbitrap, triple-quadrupole, MALDI-TOF) and liquid chromatography systems (UPLC, nanoLC).
• Develops protocols, task-specific procedures and Standard Operating Procedures (SOPs).
• Participates in proteomics projects, anticipates issues, and identifies opportunities to improve technical and team results.
• Maintains accurate records of the processing and analysis in a computerized Laboratory Information Management System (LIMS).
• Assists technical staff with safe and compliant procedures for BSL-2 laboratory access, toxin analysis of bioforensic samples, and process documentation in LIMS.
• May be appointed as a Lab Space Manager (LSM), and/or Biological Select Agents and Toxins (BSAT) designee.
• Works on assignments requiring judgment and initiative within generally defined practices and policies, while understanding the implications of work and making recommendations for solutions.
• Possesses the ability to work on multiple science tasks simultaneously, while performing assignments outside of area of expertise.
• Exercises judgment within generally defined practices and policies in selecting methods and techniques for obtaining solutions.
• Follows established procedures on routine work, requires instruction only on new assignments, and is self-sufficient in monitoring and reporting on quality, and compliance.
• Participates in the testing and evaluation of new technologies and protocols including writing and updating NBACC Standard Operating Procedures (SOPs), work instructions and forms to improve NBACC's ability to serve its customers.
• Identifies departures from the Quality Management System (QMS) and initiates actions to investigate and prevent such occurrences.
• With minimal supervision, operates, maintains, and characterizes the performance of equipment ensuring the reagents and supplies are maintained and used properly.
• Serves as a member of a team in developing, validating, and maintaining methods for identifying and characterizing biological threat agents.
• Exhibits teamwork, clear communication, openness to ideas, and patience with colleagues.
• Demonstrates the ability to multitask, maintain composure under pressure, and utilize effective time management skills to prioritize tasks in a complex, high containment environment.
• Is a self-starter driven by an eagerness to succeed, maintaining flexibility, adapting to change productively, learning new concepts, and using critical thinking to resolve complex technical problems.
• Maintains appropriate records.
• Performs other duties as assigned/authorized.

QUALIFICATIONS

• Bachelor of Science (or equivalent) in a scientific discipline and a minimum of 4 years of related experience or a Master's degree and a minimum of 2 years of related experience.

• Equivalent combination of education, training, and experience will be considered as substitution for the specific requirements outlined above.

• Experience operating mass spectrometry instrumentation such as orbitrap, triple quadrupole, and MALDI TOF.
• Proteomics and/or metabolomics experience is highly desirable.
• Experience with sample preparation techniques such as protein digestion, SDS-PAGE, solid phase extraction, and liquid-liquid extraction.
• Ability to analyze and interpret mass spectrometry data using informatics tools such as Proteome Discoverer, Compound Discoverer, MassHunter, Skyline, MaxQuant, FragPipe and MS-Dial.
• Intermediate scientific proficiency in laboratory functions. Possesses the ability to identify issues with laboratory protocols, procedures, and experimental plans.
• Proficiency and clarity of oral and written communications are essential.
• Exemplary organizational skills with a proven track record of working effectively both independently and as a team player.
• Knowledge or experience with biocontainment facilities and procedures, laboratory safety, biosurety, and decontamination is desirable.
• Skills and experience to support laboratory activities and work in accordance with NBACC's management system (e.g., ISO).

SECURITY/ELIGIBILITY REQUIREMENTS

• Must be a citizen of the United States, able to obtain and maintain an interim secret clearance leading to a top-secret clearance, suitability for DHS, and a favorable adjudication of the Department of Justice (DoJ) for select agent access.
• Participation in the Immunization Program, Medical Surveillance Program, and enrollment in the Personnel Reliability Program (PRP) is required.
• May be required to participate in NBACC's alternative work and/or on-call schedule, dependent upon business needs.