Junior Specialist - Costello lab

Junior Specialist - Costello lab

Under direct supervision, the candidate will perform standard wet-lab cell and molecular analysis on glioma cells and tissues. The candidate will also perform computational analysis of ATAC-seq and RNA-seq data.

Duties will include being trained in and assisting with tumor cell biology activities such as human cell culturing, adding transfection reagents to cell cultures, and centrifuging cells to generate a cell pellet for DNA, RNA and protein isolation. Incumbent will use a laboratory notebook and record the activities, and results such as the quantification of the cell viability, cell number and amount of material isolated from the cell pellet.

Duties will also include being trained in and assisting with tumor molecular biology activities such as polymerase chain reaction, reverse-transcription quantitative PCR, western blotting. Incumbent will be trained in and assist with pipetting molecular reagents into PCR and Eppendorf reaction tubes, heating and cooling as per laboratory protocols and recording all activities and results in a laboratory notebook. Incumbent will also have strong experience in computational analysis of ATAC-seq and RNA-seq data and use that experience to conduct computational analyses and interpret the results in the context of current literature.

Required Qualifications:

• Baccalaureate degree (or equivalent degree) or at least four years of research experience.
• The qualified applicant must have experience in conducting ATAC-seq, human cell culture, iPS cell differentiation to neuronal and oligodendrocyte progenitor cell lineages, PCR, CRISPR-based gene modulation, and some experience with xenografts of human cells into the brain of nude mice.
• The applicant must have expert hands-on capability in the computational analysis of ATAC-seq data and RNA-seq data from human brain tumors.
• The applicant must be able to perform computational analysis to discover transcription factor binding motif enrichment from open chromatin profiles of human brain tumors.

Preferred Qualifications:

• Ability to conduct unsupervised clustering, and to understand and interpret data from unsupervised covariation analysis of ATAC-seq and RNA-seq.
  
  
• In the laboratory, preferred qualifications include RNA isolation and DNA isolation.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $55,000 - $58,600.

Please apply online at https://aprecruit.ucsf.edu/JPF05762.

• 3 required (contact information only)

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.

* "Misconduct" means any violation of the policies or laws governing conduct at the applicant's previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, or discrimination, as defined by the employer.

* UC Sexual Violence and Sexual Harassment Policy

* UC Anti-Discrimination Policy

* APM - 035: Affirmative Action and Nondiscrimination in Employment